LABORTATORY DIAGNOSIS OF RUMINANTS BRUCELLAE BY AMOS PCR

Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants

Microscopy as “field based test” was compared with IS900 blood PCR for the diagnosis and estimationof prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) in domestic livestock withclinical Johne’s disease (JD). Of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood PCR, respectively. Proportional agreement between “microscopy” and blood PCR was subst...

Molecular diagnosis of Mycoplasma spp. Arthritis by PCR

Background: Arthritis is one of the most common inflammatory diseases worldwide. It is characterized by symptoms such as systemic inflammation and autoantibody production. The molecular mechanisms in pathogenesis of arthritis are not fully understood. Studies show that many microorganisms, including Mycoplasmas, play a role in arthritis. The PCR method is a fast and accurate molecular method fo...

Study of Peste Des Petits Ruminants (PPR) in Some Border Areas of Iran by Nested- PCR

In order to study on small ruminant rinderpest (PPR) in some border areas of Iran, totally 70 blood samples were taken via jugular vein from Kamyaran, Piranshahr, Lahijan, Ghorveh and Bilesavar district, in a tube with EDTA. The sampling was in the flocks located in Iran borders. The samples were sent near the ice container to laboratory. The blood samples included 28 goat and 42 sheep which to...

Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR

Neurocryptococcosis: diagnosis by PCR method.

Cryptococcus neoformans detection was optimized using PCR technique with the objective of application in the clinical laboratory diagnosis. The amplification area was ITS and 5,6S which encodes the ribosomal RNA (rRNA). A total of 72 cerebrospinal fluid (CSF) samples were used, obtained from cases with and without AIDS. The patients had cryptococcal meningitis (n = 56) and meningitis caused by ...